-         screen shots

o       Input interface

§         First screen

 

 

 

 

§         Open file – histogram file

 

 

 

 

 

 

 

§         Control Bar

·        X-axis : first 3 divided scale

·        Y-axis : the 4-th scale bar

·        Depth 1- 3 : showing the edges of relation graph

·        Color : background color – white and black

·        GeneAnnotationSave button : report the overlapped regions

 

 

 

 

§         Output control

·        VIEW tab

o       blocks

o       frequency of genome

o       COG

o       gene

o       COG/gene name

o       Link other genomes for constructing relation graph

 

·        LIST tab

o       List of edges : to get a path of some graph, click a edge in the list.

 

 

 

 

 

 

·        STAT tab

o       Average score

o       simple statistic result

 

 

 

 

 

 

 

 

·        FILTER tab

o       Block Filtering

- Setting cut_off score for making block node

 

o       Edge Filtering

- Setting range of cut_off score to connect nodes

                                           - Setting range of length of bloc node

                                           - Best hit : choose the highest score edge of all edges

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

·        Screen for filtering out nodes under edge filter

 

 

 

 

 

o       Finding site

§         Scanning an interesting site

 

 

 

 

§         Zoom out

 

 

 

 

 

 

 

 

 

 

§         Zoom out and showing the connectivity

·        1/4000 ratio

 

 

§         Showing the list of edges of relation graph

 

·        List : display the position and node number. If there is terminal and not block, the node number is -1.

 

§         Showing a path in the list of relation graph

 

 

 

 

 

§         Showing the sequences of blocks

 

 

 

 

 

§         Sample path which has different information

o       It may find new fact.

 

 

 

 

o       Reference block of genome NC_00921 has no information COG and gene, but matched blocks of the other genomes have gene information which could be find a new fact.

 

 

§         Result of multiple alignment for selected region using Clustal-w.

 

 

 

 

 

o       Annotation of new genome

 

 

 

§         If we have a new genome without any annotation, COMPAM visualizes a rough annotation.